C. trachomatis in Female Reproductive Tract Infections and RFLP-Based Genotyping: A 16-Year Study from a Tertiary Care Hospital

Presence of Chlamydia trachomatis in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay. Chlamydia antigen could be...

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Published in:Infectious Diseases in Obstetrics and Gynecology 2011, Vol.2011 (2011), p.160-165
Main Authors: Gita, Satpathy, Suneeta, Mittal, Anjana, Sharma, Niranjan, Nayak, Sujata, Mohanty, Pandey, Ravindra Mohan
Format: Article
Language:English
Subjects:
Adult
Antigens, Bacterial - genetics
Bacteria
Causes of
Chlamydia infections
Chlamydia Infections - microbiology
Chlamydia trachomatis
Chlamydia trachomatis - genetics
Clinics
Deoxyribonucleic acid
Diagnosis
DNA
Female
Genetic aspects
Genital diseases, Female
Genital Diseases, Female - microbiology
Genotype
Health aspects
Hospitals
Humans
Infections
Longitudinal Studies
Microbiology
Microscopy, Fluorescence
Middle Aged
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Porins - genetics
Restriction fragment length polymorphism analysis
Sexually transmitted diseases
STD
Womens health
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Summary:Presence of Chlamydia trachomatis in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay. Chlamydia antigen could be detected in 391 out of 2466 (15.85%) of patients studied; in 27.27% women with PID, 16.74% women with cervicitis, 16.03% women with infertility, and 12.06% women with adverse pregnancy outcomes, respectively. There was a statistically significant decreasing trend in Chlamydia antigen positivity between the years 1994–1999 and 2000–2004; the apparent decline in antigen positivity between the years 2000–2004 and 2005–2010 was not statistically significant. Antigen detection assay detected equal number of positives as the PCR assay; tissue culture isolation demonstrated lower positivity. In a few representative specimens from cervicitis patients, genotyping was done using RFLP pattern analysis of C. trachomatis MOMP gene amplified by PCR assay, all of these belonged to Chlamydia trachomatis serovar E.
ISSN:1064-7449
1098-0997
DOI:10.1155/2011/548219