The Effects of NDRG2 Overexpression on Cell Proliferation and Invasiveness of SW48 Colorectal Cancer Cell Line

Colorectal cancer (CRC) is one of the most common causes of cancer-related death in the world. The expression of N-myc downstream-regulated gene 2 (NDRG2) is down-regulated in CRC. The aim of this study was to investigate the effect of NDRG2 overexpression on cell proliferation and invasive potentia...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2015-09, Vol.40 (5), p.430-439
Main Authors: Golestan, MSc, Ali, Mojtahedi, PhD, Zahra, Ghalamfarsa, PhD, Ghasem, Hamidinia, MSc, Maryam, Takhshid, PhD, Mohammad Ali
Format: Article
Language:English
Subjects:
Analysis
Cell movement
Cell proliferation
Colorectal cancer
Colorectal neoplasms
Development and progression
Gene expression
Genetic aspects
Health aspects
Metalloproteinase
NDRG2 protein
Original
Transfection
Wound healing
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Summary:Colorectal cancer (CRC) is one of the most common causes of cancer-related death in the world. The expression of N-myc downstream-regulated gene 2 (NDRG2) is down-regulated in CRC. The aim of this study was to investigate the effect of NDRG2 overexpression on cell proliferation and invasive potential of SW48 cells. SW48 cells were transfected with a plasmid overexpressing NDRG2. After stable transfection, the effect of NDRG2 overexpression on cell proliferation was evaluated by MTT assay. The effects of NDRG2 overexpression on cell migration, invasion and cell motility and matrix metalloproteinase 9 (MMP9) activities were also investigated using matrigel transwell assay, wound healing assay and gelatin zymography, respectively. MTT assay showed that overexpression of NDRG2 caused attenuation of SW48 cell proliferation. Transwell and wound healing assay revealed that NDRG2 overexpression led to inhibition of migration, invasion, and motility of SW48 cells. The overexpression of NDRG2 also reduced the activity of secreted MMP-9. The results of this study suggest that NDRG2 overexpression inhibits proliferation and invasive potential of SW48 cells, which likely occurs via suppression of MMP-9 activity.
ISSN:0253-0716
1735-3688