Protective Effect of Castanopsis sieboldii Extract against UVB-Induced Photodamage in Keratinocytes

Ultraviolet B (UVB) rays disrupt the skin by causing photodamage via processes such as reactive oxygen species (ROS) production, endoplasmic reticulum (ER) stress, DNA damage, and/or collagen degradation. is an evergreen tree native to the southern Korean peninsula. Although it is known to have anti...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-03, Vol.28 (6), p.2842
Main Authors: Lee, Hye Rim, Yang, Ji Hye, Lee, Ji Hyun, Kim, Kyu Min, Cho, Sam Seok, Baek, Jin Sol, Kim, Jae Min, Choi, Moon-Hee, Shin, Hyun-Jae, Ki, Sung Hwan
Format: Article
Language:English
Subjects:
Acids
Aging
Antioxidants
Apoptosis
Autophagy
Butyl hydroperoxide
Castanopsis sieboldii
Cell Line
Cell viability
Collagen
Collagen - metabolism
Cytotoxicity
DNA damage
Endoplasmic reticulum
Epigallocatechin gallate
ER stress
Ethanol
Evergreen trees
Flavonoids
Inflammation
Irradiation
keratinocyte
Keratinocytes
Kinases
Molecular modelling
Oxidative stress
Physiology
Polyphenols
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Skin
Skin - metabolism
Skin injuries
Stress
Ultraviolet radiation
Ultraviolet Rays - adverse effects
UVB
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Summary:Ultraviolet B (UVB) rays disrupt the skin by causing photodamage via processes such as reactive oxygen species (ROS) production, endoplasmic reticulum (ER) stress, DNA damage, and/or collagen degradation. is an evergreen tree native to the southern Korean peninsula. Although it is known to have antioxidant and anti-inflammatory effects, its protective effect against photodamage in keratinocytes has not been investigated. Thus, in the present study, we investigated the effect of 70% ethanol extract of leaf (CSL3) on UVB-mediated skin injuries and elucidated the underlying molecular mechanisms. CSL3 treatment restored the cell viability decreased by UVB irradiation. Moreover, CSL3 significantly inhibited UVB- or tert-butyl hydroperoxide-mediated ROS generation in HaCaT cells. ER stress was inhibited, whereas autophagy was upregulated by CSL3 treatment against UVB irradiation. Additionally, CSL3 increased collagen accumulation and cell migration, which were decreased by UVB exposure. Notably, epigallocatechin gallate, the major component of CSL3, improved the cell viability decreased by UVB irradiation through regulation of ER stress and autophagy. Conclusively, CSL3 may represent a promising therapeutic candidate for the treatment of UVB-induced skin damage.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28062842