Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS

Flammulina velutipes is the fourth largest edible fungus in China with high nutritional value. In this paper, ultrahigh-performance liquid chromatography tandem hybrid quadrupole-Orbitrap mass spectrometry (UPLC-Q-Exactive-Orbitrap MS) was used to identify the secondary metabolites of F. velutipes....

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Bibliographic Details
Published in:Journal of food quality 2021, Vol.2021, p.1-8
Main Authors: Ding, Ying, Chen, Sitan, Wang, Honglin, Li, Shanlei, Ma, Changyang, Wang, Jinmei, Cui, Lili
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Apoptosis
Cancer
Chromatography
Flavonoids
Food quality
Gas flow
Ions
Ischemia
Isoflavones
Liquid chromatography
Mass spectrometry
Metabolites
Molecular weight
Nutritive value
Organic acids
Plant metabolites
Proteins
R&D
Research & development
Retention
Retention time
Scientific imaging
Secondary metabolites
Surveys
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Summary:Flammulina velutipes is the fourth largest edible fungus in China with high nutritional value. In this paper, ultrahigh-performance liquid chromatography tandem hybrid quadrupole-Orbitrap mass spectrometry (UPLC-Q-Exactive-Orbitrap MS) was used to identify the secondary metabolites of F. velutipes. The metabolites were identified by comparing the retention time, accurate molecular weight, and MS2 data with standard databases of mzVault and mzCloud (compound: 17,000+) and BGI high-resolution accurate mass plant metabolome database (plant metabolite: 2500+). Finally, 26 secondary metabolites were preliminarily identified, including flavonoids, phenylpropanoids, organic acids, and steroids.
ISSN:0146-9428
1745-4557
DOI:10.1155/2021/4103952