Comparative analysis of the circadian rhythm genes period and timeless in Culex pipiens Linnaeus, 1758 (Diptera, Culicidae)

Nucleotide sequences of the circadian rhythm genes, and , were studied for the first time in mosquitoes Linnaeus, 1758. In this work we evaluated variations of the studied genome fragments for the two forms of (forma "pipiens" - mosquitoes common for aboveground habitats, forma "moles...

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Bibliographic Details
Published in:Comparative cytogenetics 2016-01, Vol.10 (4), p.483-504
Main Authors: Shaikevich, Elena V, Karan, Ludmila S, Fyodorova, Marina V
Format: Article
Language:English
Subjects:
Acids
Adaptation
Aquatic insects
Circadian rhythm
Circadian rhythms
Comparative analysis
Culex pipiens
Culicidae
DNA fingerprints
Gene sequencing
Genes
Genetic markers
Genomes
Mosquitoes
Mutation
Natural selection
Phylogeny
Population genetics
Proteins
Timeless protein
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Summary:Nucleotide sequences of the circadian rhythm genes, and , were studied for the first time in mosquitoes Linnaeus, 1758. In this work we evaluated variations of the studied genome fragments for the two forms of (forma "pipiens" - mosquitoes common for aboveground habitats, forma "molestus" - underground mosquitoes). We compared from Russia with transatlantic and subtropical Say, 1823. Our results show that intraspecies variability is higher for the gene than for the gene . The revealed substitutions in nucleotide sequences and especially in amino acid sequences grouped the individuals of the two forms into distinct clusters with high significance. The detected fixed amino acid substitutions may appear essential for functioning of the circadian rhythm proteins in , and may be correlated with adaptations of the taxa within the group . Our results suggest that natural selection favors fixed mutations and the decrease in diversity of the genes and in mosquitoes of the Culex pipiens f. "molestus" compared with the Culex pipiens f. "pipiens", is probably correlated with adaptive features of Culex pipiens f. "molestus". The studied genome regions may be considered as promising molecular-genetic markers for identification, population and phylogenetic analysis of similar species and forms of the complex.
ISSN:1993-0771
1993-078X
DOI:10.3897/CompCytogen.v10i4.7582