Quantitative Trait Locus Analysis of Seed Germination and Seedling Vigor in Brassica rapa Reveals QTL Hotspots and Epistatic Interactions

The genetic basis of seed germination and seedling vigor is largely unknown in Brassica species. We performed a study to evaluate the genetic basis of these important traits in a B. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded vegetable-ty...

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Bibliographic Details
Published in:Frontiers in plant science 2015-12, Vol.6, p.1032-1032
Main Authors: Basnet, Ram K, Duwal, Anita, Tiwari, Dev N, Xiao, Dong, Monakhos, Sokrat, Bucher, Johan, Visser, Richard G F, Groot, Steven P C, Bonnema, Guusje, Maliepaard, Chris
Format: Article
Language:English
Subjects:
BIOS Plant Development Systems
Brassica rapa
candidate genes
EPS
Laboratorium voor Plantenveredeling
Laboratory of Plant Breeding
PBR Breeding for growth and development
PBR Groei & Ontwikkeling
PBR Kwantitatieve Aspecten
PBR Quantitative aspects of Plant Breeding
PE&RC
Plant Breeding
Plant Science
PRI BIOS Plant Development Systems
QTL mapping
salt stress
seed germination
seedling vigour
WUR PB Groei & Ontwikkeling
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Summary:The genetic basis of seed germination and seedling vigor is largely unknown in Brassica species. We performed a study to evaluate the genetic basis of these important traits in a B. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded vegetable-type pak choi. We identified 26 QTL regions across all 10 linkage groups for traits related to seed weight, seed germination and seedling vigor under non-stress and salt stress conditions illustrating the polygenic nature of these traits. QTLs for multiple traits co-localized and we identified eight hotspots for quantitative trait loci (QTL) of seed weight, seed germination, and root and shoot lengths. A QTL hotspot for seed germination on A02 mapped at the B. rapa Flowering Locus C (BrFLC2). Another hotspot on A05 with salt stress specific QTLs co-located with the B. rapa Fatty acid desaturase 2 (BrFAD2) locus. Epistatic interactions were observed between QTL hotspots for seed germination on A02 and A10 and with a salt tolerance QTL on A05. These results contribute to the understanding of the genetics of seed quality and seeding vigor in B. rapa and can offer tools for Brassica breeding.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2015.01032