Relationship between the invasion of lymphocytes and cytokines in the tumor microenvironment and the interval after single brachytherapy hypofractionated radiotherapy and conventional fractionation radiotherapy in non-small cell lung Cancer

• Published in: BMC cancer 2020-09, Vol.20 (1), p.893-14, Article 893
• Main Authors: Li, Lin, Yue, Hong Cheng, Han, Yun Wei, Liu, Wei, Xiong, Liang Geng, Zhang, Jian Wen
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibodies; Apoptosis; Biological response modifiers; Brachytherapy; Brachytherapy - methods; Cancer therapies; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Non-Small-Cell Lung - radiotherapy; CD4 antigen; CD8 antigen; CD86 antigen; Cell Line, Tumor; Conventional fractionation radiotherapy; Cytokine; Cytokines; Dendritic cells; Disease Models, Animal; Enzyme-linked immunosorbent assay; Enzymes; Experiments; Female; Flow cytometry; Foxp3 protein; Growth inhibition; Humans; Hypofractionated radiotherapy; Immunohistochemistry; Immunotherapy; Interferon; Interleukin 10; Interleukin 12; Interleukins; Laboratory animals; Lung cancer; Lung Neoplasms - pathology; Lung Neoplasms - radiotherapy; Lymphocyte; Lymphocytes; Lymphocytes - metabolism; Medical laboratories; Mice; Necrosis; Non-small cell lung cancer; Non-small cell lung carcinoma; Physiological aspects; Radiation therapy; Reagents; Small cell lung cancer; Small cell lung carcinoma; Tomography; Tumor Microenvironment; Tumors; γ-Interferon
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-020-07403-1

The effect of brachytherapy on lymphocytes and cytokines in the tumor microenvironment is unclear. This study aimed to analyze the relationship between the invasion of lymphocytes and cytokines in the tumor microenvironment and the interval after single brachytherapy hypofractionated radiotherapy (SBHFRT) and conventional fractionation radiotherapy (CFRT) in non-small cell lung cancer (NSCLC). Lewis tumor-bearing mice were randomly divided into control, CFRT, and SBHFRT groups. On days 7 and 14 after radiation, the expression levels of CD86 , CD4 , CD8 , and Foxp3 cells, and levels of Ki-67 protein were detected by immunohistochemistry, and the tumor necrosis rate was calculated. Following this, the levels of interleukin-10 (IL-10), IL-12, and interferon-γ (IFN-γ) were detected by enzyme-linked immunosorbent assay. The apoptosis rate was evaluated via flow cytometry. The tumor volume and tumor growth inhibition rate (TGIR) were calculated on day 14. Tumor metabolism was assessed via 18F-FDG micropositron emission tomography/computer tomography. The tumor volume reduced by 22.0% and TGIR increased by 92.2% (p