The GA and ABA signaling is required for hydrogen-mediated seed germination in wax gourd

• Published in: BMC plant biology 2024-06, Vol.24 (1), p.542-15
• Main Authors: Chang, Jingjing, Li, Jiawei, Li, Jinlong, Chen, Xiao, Jiao, Jiabin, Li, Jing, Song, Zhao, Zhang, Baige
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - metabolism; Agricultural research; Biosynthesis; Catabolism; Chemical properties; Environmental aspects; Ethylene; Gene expression; Gene Expression Regulation, Plant - drug effects; Genes; Germination; Germination - drug effects; Gibberellin; Gibberellins; Gibberellins - metabolism; Gourds; Hydrogen; Hydrogen - metabolism; Hydrogen-rich water (HRW); Kinases; Metabolism; Physiological aspects; Physiological effects; Physiology; Plant growth; Plant Growth Regulators - metabolism; Regulation; Seed germination; Seeds; Seeds - drug effects; Seeds - genetics; Seeds - growth & development; Seeds - physiology; Signal Transduction; Sodium tungstate; Transcriptomes; Tungsten compounds; Wax Gourd; Waxes
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05193-3

Hydrogen gas (H ), a novel and beneficial gaseous molecule, plays a significant role in plant growth and development processes. Hydrogen-rich water (HRW) is regarded as a safe and easily available way to study the physiological effects of H on plants. Several recent research has shown that HRW attenuates stress-induced seed germination inhibition; however, the underlying modes of HRW on seed germination remain obscure under non-stress condition. In this current study, we investigated the possible roles of gibberellin (GA) and abscisic acid (ABA) in HRW-regulated seed germination in wax gourd (Benincasa hispida) through pharmacological, physiological, and transcriptome approaches. The results showed that HRW application at an optimal dose (50% HRW) significantly promoted seed germination and shortened the average germination time (AGT). Subsequent results suggested that 50% HRW treatment stimulated GA production by regulating GA biosynthesis genes (BhiGA3ox, BhiGA2ox, and BhiKAO), whereas it had no effect on the content of ABA and the expression of its biosynthesis (BhiNCED6) and catabolism genes (BhiCYP707A2) but decreased the expression of ABA receptor gene (BhiPYL). In addition, inhibition of GA production by paclobutrazol (PAC) could block the HRW-mediated germination. Treatment with ABA could hinder HRW-mediated seed germination and the ABA biosynthesis inhibitor sodium tungstate (ST) could recover the function of HRW. Furthermore, RNA-seq analysis revealed that, in the presence of GA or ABA, an abundance of genes involved in GA, ABA, and ethylene signal sensing and transduction might involve in HRW-regulated germination. This study portrays insights into the mechanism of HRW-mediated seed germination, suggesting that HRW can regulate the balance between GA and ABA to mediate seed germination through ethylene signals in wax gourd.