Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial

Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly and , with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of and in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consis...

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Published in:Gels 2023-02, Vol.9 (2), p.117
Main Authors: Pessanha, Fernanda Soares, Oliveira, Beatriz Guitton Renaud Baptista de, Oliveira, Bianca Campos, Deutsch, Gabriela, Teixeira, Felipe Lopes, Bokehi, Luciana Castilho, Calomino, Mariana Alcântara, Rodrigues de Castilho, Selma, Thiré, Rossana Mara da Silva Moreira, Teixeira, Lenise Arneiro, Paula, Geraldo Renato de
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics
Antimicrobial agents
biofilm
Biofilms
Cellulose
Clinical trials
Debridement
Diabetes
Diabetes mellitus
Diabetic foot
Diabetics
Epidermal growth factor
Fibroblasts
Growth factors
Hydrogels
Microorganisms
Patients
Penicillin
Product development
Pseudomonas aeruginosa
Staphylococcus aureus
Staphylococcus aureus infections
Type 2 diabetes
Ulcers
Virulence
Virulence (Microbiology)
Wound healing
Wounds and injuries
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Summary:Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly and , with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of and in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 strains and 15 strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels9020117