Freedom to operate analysis, design and evaluation of expression cassettes that confer tolerance to glyphosate

Tolerance to the herbicide glyphosate is the most extended feature in commercial transgenic events released worldwide and it is an example of the successful use of genetic modification to improve weed control in crops. Glyphosate-tolerant genotypes have been developed by multinational corporations u...

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Bibliographic Details
Published in:Agronomía colombiana 2020-05, Vol.38 (2), p.161-170
Main Authors: Jiménez-Barreto, Jenny, Mora-Oberlaender, Julián, Chaparro-Giraldo, Alejandro
Format: Article
Language:English
Subjects:
AGRONOMY
Amino acids
Cassettes
codon usage
Commercialization
Design
Enzymes
gene design
Genetic modification
Genetic transformation
Genetically altered foods
Genomes
Genotypes
Glycine max
Glyphosate
herbicide tolerance
Herbicides
Intellectual property
Multinational corporations
Nicotiana benthamiana
Peptides
Polymerase chain reaction
Research facilities
Research institutions
Soybeans
Transgenic plants
Weed control
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Summary:Tolerance to the herbicide glyphosate is the most extended feature in commercial transgenic events released worldwide and it is an example of the successful use of genetic modification to improve weed control in crops. Glyphosate-tolerant genotypes have been developed by multinational corporations using patent- protected technologies. For some of these events, all associated patents have expired and, therefore, have become a good target for the development of herbicide-tolerant agbiogenerics by national research institutions, using local crop varieties. As a first step in this process, we present the design (in silico) of three expression cassettes with the purpose of using them in the transformation of Colombian soybean (Glycine max) varieties to confer them tolerance to glyphosate. We transformed Nicotiana benthamiana as a model to validate the functionality of the expression cassettes and detected the expression of the transgene by RT-PCR. Additionally, a Freedom to Operate analysis of the sequences used in the expression cassettes suggests that their commercial use in Colombia does not infringe third party rights. This analysis must be updated and validated by intellectual property experts prior to commercialization.
ISSN:0120-9965
2357-3732
DOI:10.15446/agron.colomb.v38n2.79150