Overexpression of OsRbohH Enhances Heat and Drought Tolerance through ROS Homeostasis and ABA Mediated Pathways in Rice ( Oryza sativa L.)

Respiratory burst oxidase homologs (Rbohs) are the primary producers of reactive oxygen species (ROS), which have been demonstrated to play critical roles in plant responses to abiotic stress. Here, we explored the function of in heat and drought stress tolerance by generating overexpression lines (...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2024-09, Vol.13 (17), p.2494
Main Authors: Chen, Yating, Zhang, Rui, Wang, Rujie, Li, Jiangdi, Wu, Bin, Zhang, Haiwen, Xiao, Guiqing
Format: Article
Language:English
Subjects:
ABA
Abiotic stress
Abscisic acid
Antioxidants
Catalase
Chlorophyll
Drought
Drought resistance
drought stress
Electrolyte leakage
Enzymes
Genes
Gibberellins
Heat
heat tolerance
Homeostasis
Nitric oxide
OsRbohH
Peroxidase
Plant layout
Reactive oxygen species
Respiratory burst oxidase
rice
ROS
Survival
Transgenic plants
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Respiratory burst oxidase homologs (Rbohs) are the primary producers of reactive oxygen species (ROS), which have been demonstrated to play critical roles in plant responses to abiotic stress. Here, we explored the function of in heat and drought stress tolerance by generating overexpression lines ( -OE). was highly induced by various abiotic stress and hormone treatments. Compared to wild-type (WT) controls, -OE plants exhibited enhanced tolerance to heat and drought, as determined by survival rate analyses and total chlorophyll content. Histochemical staining revealed that -OE accumulated less ROS. This is consistent with the observed increase in catalase (CAT) and peroxidase (POD) activities, as well as a reduced electrolyte leakage rate and malondialdehyde (MDA) content. Moreover, -OE exhibited enhanced sensitivity to exogenous abscisic acid (ABA), accompanied by altered expression levels of ABA synthesis and catabolic genes. Further analysis indicated that transgenic lines had lower transcripts of ABA signaling-related genes ( , , and ) under heat but higher levels under drought than WT. In conclusion, these results suggest that is a positive regulator of heat and drought tolerance in rice, which is probably performed through OsRbohH-mediated ROS homeostasis and ABA signaling.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13172494