Influence of Post-Milking Treatment on Microbial Diversity on the Cow Teat Skin and in Milk

• Published in: Dairy (Basel) 2022-06, Vol.3 (2), p.262-276
• Main Authors: Verdier-Metz, Isabelle, Delbès, Céline, Bouchon, Matthieu, Pradel, Philippe, Theil, Sébastien, Rifa, Etienne, Corbin, Agnès, Chassard, Christophe
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Animal biology; Animal lactation; Bacteria; bacterial community; Cattle; Chlorhexidine; Dairy cattle; dairy cow; Disinfection; Disinfection & disinfectants; Emulsions; Food engineering; fungal community; Gene sequencing; Lactic acid; Lactic acid bacteria; Life Sciences; Microbial activity; Microbiology and Parasitology; Microbiota; Microorganisms; Milk; Milking; Next-generation sequencing; Pasture; Population; Population studies; post-milking; Skin; teat; Veterinary medicine and animal Health; Yeasts; Zootechny
• ISSN: 2624-862X; 2624-5647; 2624-862X; 2624-5647
• DOI: 10.3390/dairy3020021

In dairy cattle, teat disinfection at the end of milking is commonly applied to limit colonization of the milk by pathogenic microorganisms via the teat canal. The post-milking products used can irritate the teat skin and unbalance its microbial population. Our study aimed to assess the impact of different milking products on the balance of the microbial communities on the teat skin of cows and in their milk. For 12 weeks at the end of each milking operation, three groups of seven Holstein dairy cows on pasture received either a chlorhexidine gluconate-based product (G) or a hydrocolloidal water-in-oil emulsion (A), or no post-milking product (C). The composition of the bacterial and fungal communities on the teat skin and in the milk were characterized using a culture-dependent method and by high-throughput sequencing of marker genes to obtain amplicon sequence variants (ASVs). The individual microbiota on the cows’ teat skin was compared for the first time to that of a cow pool. In contrast to the milk, the post-milking treatment influenced the microbiota of the teat skin, which revealed a high microbial diversity. The water-in-oil emulsion appeared to slightly favour lactic acid bacteria and yeasts and to limit the development of undesirable bacteria such as Pseudomonas and Staphylococcus.