Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice

• Published in: Journal of ayurveda and integrative medicine 2021-04, Vol.12 (2), p.245-253
• Main Authors: Christina, Yuyun Ika, Diana, Meyla Restia, Fuzianingsih, Eka Noviya, Nurhayati, Ridwan, Faradisa Noviani, Widodo, Rifa’i, Muhaimin, Djati, Muhammad Sasmito
• Format: Article
• Language: English
• Subjects: E. coli; Elephantopus scaber; Hormonal; Original (Experimental); Pregnant; Sauropus androgynous
• ISSN: 0975-9476; 0976-2809
• DOI: 10.1016/j.jaim.2020.09.001

Elephantopus scaber (ES) and Sauropus androgynous (SA) have been frequently reported to possess antibacterial activity through in vitro, but in vivo studies about the protective effect of combined ES and SA have acquired less attention. To evaluate protective effect of combined ethanol extract of ES and SA on hormone imbalance and renal and hepatic necrosis formation in Escherichia coli-infected pregnant mice. A total of 28 pregnant Balb/c mice were divided into seven groups (n = 4): control, E. coli-infected pregnant mice, infected pregnant mice received 200 mg/kg ES, infected pregnant mice received combined 150 mg/kg ES and 37.5 mg/kg SA (75:25), 100 mg/kg ES and 75 mg/kg SA (50:50), 50 mg/kg ES and 112.5 mg/kg SA (25:75), and only 150 mg/kg SA. Pregnant mice were orally treated with combined ES and SA on day 1–4th of pregnancy. On the 4th day, mice were infected with 107 CFU/mL of E. coli and continuously treated with ES and SA until the 16th day of pregnancy. After treatment, the kidney and liver were prepared for histological examination using H&E staining. The blood serum was collected in each stage of pregnancy and measured by ELISA assays. Combined ES and SA gave an impact on altering the prolactin level. Combined ES and SA at ratio dose 75:25 was able to restore progesterone to normal levels (P