Magnetic beads-based nanozyme for portable colorimetric biosensing of Helicobacter pylori

• Published in: Biosensors and bioelectronics. X 2024-10, Vol.20, p.100517, Article 100517
• Main Authors: Suaifan, Ghadeer A.R.Y., Shehadeh, Mayadah B., Darwish, Rula M., Alterify, Manar, Abu Jbara, Ward, Abu Jbara, Fahid, Alaridah, Nader, Zourob, Mohammed
• Format: Article
• Language: English
• Subjects: Colorimetric; Diagnostic; Environmental contaminants; Gastric carcinoma; Lab on a chip; Sensor
• ISSN: 2590-1370; 2590-1370
• DOI: 10.1016/j.biosx.2024.100517

Cancer continues to be a significant global health issue with one in six deaths linked to the disease despite advancements in cancer detection and treatment. Recently, Helicobacter pylori (H. pylori) was identified as a risk factor for cancer development. This gram-negative bacterium is associated with gastric conditions, including stomach cancer. Although the exact transmission methods of this bacterium are still unclear, studies suggest that waterborne transmission is possible. This study focuses on the development of a colorimetric nanomaterial-based paper biosensor for specific H. pylori detection using H. pylori extracellular proteases as biomarkers. The biosensor utilizes a unique substrate labeled with magnetic nanobeads and bound to a gold sensing platform. The biosensor's limit of detection (LOD) of 100 CFU/mL, selectivity, stability, and ability to detect H. pylori in clinical specimens were evaluated, demonstrating promising results in terms of sensitivity and specificity. In comparison to traditional methods, this biosensor offers advantages in simplicity and ease of use, making it appropriate for on-site detection in both environmental and clinical settings. •Development of colorimetric H. pylori sensor probe using extracellular proteases as biomarkers.•Colorimetric biosensor illustrated a lower Limit of Detection of 100 CFU/mL.•H. pylori sensor probe selectively detected H. pylori from other food poisoning pathogens such as S. aureus and E. coli.•Colorimetric biosensors offer fast response and convenience to end-users.•Point-of-care biosensors are exploited for robust performance in H. pylori detection in environmental and clinical samples.