Ultrasound assisted wall-breaking extraction and primary structures, bioactivities, rheological properties of novel Exidia yadongensis polysaccharide

• Published in: Ultrasonics sonochemistry 2023-12, Vol.101, p.106643-106643, Article 106643
• Main Authors: Tang, Ying, Miao, Yuzhi, Tan, Min, Ma, Qinqin, Liu, Chengyi, Yang, Mei, Su, Yanqiu, Li, Qi
• Format: Article
• Language: English
• Subjects: Antioxidants - chemistry; Bioactivities; Chemical Fractionation - methods; Exidia yadongensis polysaccharide; Original; Polysaccharides - chemistry; Primary structure; Response surface; Rheological properties; Rheology; Spectroscopy, Fourier Transform Infrared; Ultrasound extraction
• ISSN: 1350-4177; 1873-2828
• DOI: 10.1016/j.ultsonch.2023.106643

New natural multifunctional polysaccharide and its innovatory extraction technology may be urgently needed for food industries. Our aims were to establish new extraction method and investigate the primary structures, bioactivities and rheological properties of novel E. yadongensis polysaccharide (EYP). Ultrasound assisted mechanical wall-breaking extraction (MAUE) was successfully established for the EYP extraction from a new E. yadongensis. Based on the MAUE with RSM, the polysaccharide yield of 17.92 ± 0.56 % with the optimal parameters of five extraction factors were obtained, and current MAUE was characterized by its high yield, low extraction temperature and short ultrasound time. After the isolation and purification, the EYP as a protein-bound polysaccharide was obtained. FT-IR and NMR analysis showed that the main backbone of the EYP comprised of (1 → 4)-β-D-glucopyranosyl and (1 → 6)-ɑ-D-mannopyranosyl groups; EYP exhibited significant antioxidant, antibacterial, antitumor, antidiabetic activities, and good viscoelastic properties in low pH solutions (P