Toxigenic and non-toxigenic patterns I, II and III and biofilm-forming ability in Bacteroides fragilis strains isolated from patients diagnosed with colorectal cancer

• Published in: Gut pathogens 2020-06, Vol.12 (1), p.28-7, Article 28
• Main Authors: Jasemi, Seyedesomaye, Emaneini, Mohammad, Fazeli, Mohammad Sadegh, Ahmadinejad, Zahra, Nomanpour, Bizhan, Sadeghpour Heravi, Fatemah, Sechi, Leonardo A, Feizabadi, Mohammad Mehdi
• Format: Article
• Language: English
• Subjects: Abundance; Bacteria; Bacteroides fragilis; bft gene; Biofilm; Biofilms; Biopsy; Cancer; Cancer patients; Colorectal cancer; Colorectal carcinoma; CRC; Culture media; Development and progression; Enterotoxigenic Bacteroides fragilis; ETBF; Gene expression; Genes; Health aspects; Low density lipoproteins; Pathogenicity; Patients; Studies; Virulence factors
• ISSN: 1757-4749; 1757-4749
• DOI: 10.1186/s13099-020-00366-5

(ETBF) associated with the initiation and progression of colorectal cancer (CRC) has been alarmingly reported all over the world. In this study, simultaneous investigation of toxigenic and non-toxigenic patterns I, II and III and biofilm formation ability of isolated from patients with colorectal cancer was performed. Thirty-one patients diagnosed with CRC and thirty-one control subjects were recruited in this study. Specimens were cultured on BBE and BBA culture media. Classical phenotypic identification tests and PCR was performed to verify presence. Also, biofilm-forming ability and expression of gene were assessed under biofilm and planktonic forms. A total of 68 was isolated from all colorectal tissue, of which 13 isolates (19.1%) (11 isolates from CRC and 2 from normal tissue) were positive for gene. The abundance patterns of I, II and III were as follow in descending order; pattern I > pattern III > pattern II in CRC subjects and pattern II > pattern III > pattern I in normal tissues. Also, pattern I showed higher biofilm formation ability compared to other patterns. Toxin expression was significantly reduced in biofilm form comparing with planktonic form. Based on our findings, there was a difference between the abundance of patterns I, II, and III and biofilm formation in isolates obtained from CRC and normal tissues. Biofilm formation ability and toxin encoding gene ( ) are two main virulence factors in pathogenicity which require more investigation to treat infections effectively.