A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii

• Published in: STAR protocols 2022-09, Vol.3 (3), p.101640-101640, Article 101640
• Main Authors: Elman, Tamar, Yacoby, Iftach
• Format: Article
• Language: English
• Subjects: Biotechnology and bioengineering; Energy; Microbiology; Model organisms; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2022.101640

H2 production from green-microalgae, for energy purposes, is the ultimate goal of large-scale production. Here, we present a two-phase protocol for hydrogen production assay under ambient conditions using Chlamydomonas reinhardtii, which eliminates steps used previously, including centrifugation and resuspension with sulfur-deprived media. We detail steps for Chlamydomonas reinhardtii culture, acetate supply replenishment, anaerobic induction, and H2 quantification. This protocol enables large-scale experiments in an easy and cost-effective method while maintaining cells vital, crucial factors for transition to industrial scales. For complete details on the use and execution of this protocol, please refer to Elman et al. (2022). [Display omitted] •2-step protocol for ambient hydrogen production in green algae•Detailed culture steps of Chlamydomonas reinhardtii•Acetate supply via addition of glacial acetic acid as a carbon source•H2 production assay without centrifugation or sulfur-deprivation requirements Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. H2 production from green-microalgae, for energy purposes, is the ultimate goal of large-scale production. Here, we present a two-phase protocol for hydrogen production assay under ambient conditions using Chlamydomonas reinhardtii, which eliminates steps used previously, including centrifugation and resuspension with sulfur-deprived media. We detail steps for Chlamydomonas reinhardtii culture, acetate supply replenishment, anaerobic induction, and H2 quantification. This protocol enables large-scale experiments in an easy and cost-effective method while maintaining cells vital, crucial factors for transition to industrial scales.