Uncovering the impact of UV radiation on mitochondria in dermal cells: a STED nanoscopy study

Mitochondria are essential organelles in cellular energy metabolism and other cellular functions. Mitochondrial dysfunction is closely linked to cellular damage and can potentially contribute to the aging process. The purpose of this study was to investigate the subcellular structure of mitochondria...

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Published in:Scientific reports 2024-04, Vol.14 (1), p.8675-8675, Article 8675
Main Authors: Kim, Hyung Jun, Jin, Seon-Pil, Kang, Jooyoun, Bae, So Hyeon, Son, Jung Bae, Oh, Jang-Hee, Youn, Hyewon, Kim, Seong Keun, Kang, Keon Wook, Chung, Jin Ho
Format: Article
Language:English
Subjects:
631/61/350
631/80/2373
Aging
Animals
Confocal microscopy
Energy metabolism
HeLa Cells
Humanities and Social Sciences
Humans
Mice
Microscopy, Fluorescence - methods
Mitochondria
Mitochondria - metabolism
Mitochondrial Membranes - metabolism
multidisciplinary
Nucleoids
Organelles
Science
Science (multidisciplinary)
Translocase
Ultraviolet radiation
Ultraviolet Rays
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Summary:Mitochondria are essential organelles in cellular energy metabolism and other cellular functions. Mitochondrial dysfunction is closely linked to cellular damage and can potentially contribute to the aging process. The purpose of this study was to investigate the subcellular structure of mitochondria and their activities in various cellular environments using super-resolution stimulated emission depletion (STED) nanoscopy. We examined the morphological dispersion of mitochondria below the diffraction limit in sub-cultured human primary skin fibroblasts and mouse skin tissues. Confocal microscopy provides only the overall morphology of the mitochondrial membrane and an indiscerptible location of nucleoids within the diffraction limit. Conversely, super-resolution STED nanoscopy allowed us to resolve the nanoscale distribution of translocase clusters on the mitochondrial outer membrane and accurately quantify the number of nucleoids per cell in each sample. Comparable results were obtained by analyzing the translocase distribution in the mouse tissues. Furthermore, we precisely and quantitatively analyzed biomolecular distribution in nucleoids, such as the mitochondrial transcription factor A (TFAM), using STED nanoscopy. Our findings highlight the efficacy of super-resolution fluorescence imaging in quantifying aging-related changes on the mitochondrial sub-structure in cells and tissues.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-55778-z