Optimizing Sample Size for Population Genomic Study in a Global Invasive Lady Beetle, Harmonia Axyridis

Finding optimal sample sizes is critical for the accurate estimation of genetic diversity of large invasive populations. Based on previous studies, we hypothesized that a minimal sample size of 3-8 individuals is sufficient to dissect the population architecture of the harlequin lady beetle, , a bio...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2020-05, Vol.11 (5), p.290
Main Authors: Li, Hongran, Qu, Wanmei, Obrycki, John J, Meng, Ling, Zhou, Xuguo, Chu, Dong, Li, Baoping
Format: Article
Language:English
Subjects:
2b-RAD
Biological control
Deoxyribonucleic acid
DNA
DNA sequencing
empirical investigation
Endonuclease
Enzymes
Genetic diversity
Genomes
Harmonia axyridis
Introduced species
Invasive species
Nonnative species
Nucleotides
Optimization
Phylogenetics
Population
Population genetics
population genomics
Population studies
Populations
Sample size
Single-nucleotide polymorphism
Software
Studies
Tactics
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Summary:Finding optimal sample sizes is critical for the accurate estimation of genetic diversity of large invasive populations. Based on previous studies, we hypothesized that a minimal sample size of 3-8 individuals is sufficient to dissect the population architecture of the harlequin lady beetle, , a biological control agent and an invasive alien species. Here, equipped with a type IIB endonuclease restriction site-associated (2b-RAD) DNA sequencing approach, we identified 13,766 and 13,929 single nucleotide polymorphisms (SNPs), respectively, among native and invasive populations. With this information we simulated populations using a randomly selected 3000 SNPs and a subset of individuals. From this simulation we finally determined that six individuals is the minimum sample size required for the accurate estimation of intra- and inter-population genetic diversity within and across populations. Our findings provide an empirical advantage for population genomic studies of in particular and suggest useful tactics for similar studies on multicellular organisms in general.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects11050290