Long-term nitrogen deposition enhances microbial capacities in soil carbon stabilization but reduces network complexity

• Published in: Microbiome 2022-07, Vol.10 (1), p.1-112, Article 112
• Main Authors: Ma, Xingyu, Wang, Tengxu, Shi, Zhou, Chiariello, Nona R., Docherty, Kathryn, Field, Christopher B., Gutknecht, Jessica, Gao, Qun, Gu, Yunfu, Guo, Xue, Hungate, Bruce A., Lei, Jiesi, Niboyet, Audrey, Le Roux, Xavier, Yuan, Mengting, Yuan, Tong, Zhou, Jizhong, Yang, Yunfeng
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Ammonia; BASIC BIOLOGICAL SCIENCES; Carbon; Chitinase; Copy number; Data processing; Degradation; Ecology, environment; Ecosystems; Environmental Sciences; Enzymatic activity; Experiments; Genes; GeoChip; Global change; Global Changes; Grasslands; High-throughput sequencing; Hybridization; Life Sciences; Microbiology and Parasitology; Mineralization; Nitrates; Nitrogen; Nitrogen deposition; Phenols; Precipitation; Relative abundance; rRNA; Soil microbial community; Soil study; Taxonomy; Terrestrial ecosystems
• ISSN: 2049-2618; 2049-2618
• DOI: 10.1186/s40168-022-01309-9

Background Anthropogenic activities have increased the inputs of atmospheric reactive nitrogen (N) into terrestrial ecosystems, affecting soil carbon stability and microbial communities. Previous studies have primarily examined the effects of nitrogen deposition on microbial taxonomy, enzymatic activities, and functional processes. Here, we examined various functional traits of soil microbial communities and how these traits are interrelated in a Mediterranean-type grassland administrated with 14 years of 7 g m−2 year−1 of N amendment, based on estimated atmospheric N deposition in areas within California, USA, by the end of the twenty-first century. Results Soil microbial communities were significantly altered by N deposition. Consistent with higher aboveground plant biomass and litter, fast-growing bacteria, assessed by abundance-weighted average rRNA operon copy number, were favored in N deposited soils. The relative abundances of genes associated with labile carbon (C) degradation (e.g., amyA and cda) were also increased. In contrast, the relative abundances of functional genes associated with the degradation of more recalcitrant C (e.g., mannanase and chitinase) were either unchanged or decreased. Compared with the ambient control, N deposition significantly reduced network complexity, such as average degree and connectedness. The network for N deposited samples contained only genes associated with C degradation, suggesting that C degradation genes became more intensely connected under N deposition. Conclusions We propose a conceptual model to summarize the mechanisms of how changes in above- and belowground ecosystems by long-term N deposition collectively lead to more soil C accumulation. Video Abstract