Theileria terrestris nov. sp.: A Novel Theileria in Lowland Tapirs ( Tapirus terrestris ) from Two Different Biomes in Brazil

The low-land tapir ( ) is the largest wild terrestrial mammal found in Brazil. Although has been already reported as a host of hemoparasites, the occurrence and genetic identity of Piroplasmida agents in this species is still cloudy. Although it is reported that , an endemic equid-infective agent in...

Full description

Saved in:
Bibliographic Details
Published in:Microorganisms (Basel) 2022-11, Vol.10 (12), p.2319
Main Authors: Mongruel, Anna Claudia Baumel, Medici, Emília Patrícia, da Costa Canena, Ariel, Calchi, Ana Cláudia, Perles, Lívia, Rodrigues, Bianca Cardenal Balla, Soares, João Fabio, Machado, Rosangela Zacarias, André, Marcos Rogério
Format: Article
Language:English
Subjects:
Animals
Aquariums
Cerrado
Disease transmission
Ecosystems
EMA-1 gene
Fragments
Gene sequencing
Genes
Hsp70 protein
Investigations
New species
Pantanal
Pathogenicity
Pathogens
Phylogenetics
Phylogeny
Piroplasmida
Polymerase chain reaction
rRNA 18S
Software
Tapiridae
tapirs
Tapirus terrestris
Theileria
theileriosis
Topology
Vectors
Vertebrates
wildlife
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The low-land tapir ( ) is the largest wild terrestrial mammal found in Brazil. Although has been already reported as a host of hemoparasites, the occurrence and genetic identity of Piroplasmida agents in this species is still cloudy. Although it is reported that , an endemic equid-infective agent in Brazil, is occurring in lowland tapirs, these reports are probably misconceived diagnoses since they are solely based on small fragments of 18S rRNA that may not achieve accurate topologies on phylogenetic analyses. The present study aimed to detect and investigate the identity of spp. in tapirs from Pantanal and Cerrado biomes. Blood-DNA samples from tapirs were screened for a partial (~800 bp) 18S rRNA gene fragment from Piroplasmida and 64 (64/122; 52.46% CI: 43.66-61.11%) presented bands of expected size. Samples were submitted to different protocols for molecular characterization, including near-full length 18S rRNA gene (~1500 bp), and the gene from . Eight sequences were obtained for extended fragments (1182-1473 bp) from the 18S rRNA gene. Moreover, three sequences from partial and five from partial gene were obtained. None of the samples presented amplifications for the gene. Phylogenetic and distance analyses from the 18S rRNA sequences obtained demonstrated a clear separation from tapirs' spp. and . Phylogenetic analyses of and sequences obtained herein also showed a unique clade formed by tapir's spp. sp. nov. is positioned apart from all other species in 18S rRNA, , and phylogenetic analyses. This novel proposed species represents a new Piroplasmida clade, yet to be characterized regarding biological features, vectors involved in the transmission cycles, additional vertebrate hosts, and pathogenicity.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10122319