Salicylic Acid Treatment Alleviates the Heat Stress Response by Reducing the Intracellular ROS Level and Increasing the Cytosolic Trehalose Content in Pleurotus ostreatus

• Published in: Microbiology spectrum 2023-02, Vol.11 (1), p.e0311322-e0311322
• Main Authors: Zhang, Guang, Yan, Peng, Leng, Doudou, Shang, Li, Zhang, Chaohui, Wu, Zhongwei, Wang, Zhenhe
• Format: Article
• Language: English
• Subjects: Ca2; Calcium Chloride - metabolism; Food Microbiology; heat stress; Heat-Shock Response - physiology; Hormones - metabolism; Hydrogen Peroxide - metabolism; Pleurotus - metabolism; Reactive Oxygen Species - metabolism; Research Article; ROS; salicylic acid; Salicylic Acid - metabolism; Salicylic Acid - pharmacology; Trehalose
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.03113-22

Pleurotus ostreatus is usually cultivated in horticultural facilities that lack environmental control systems and often suffer heat stress (HS). Salicylic acid (SA) is recognized as a plant defense-related hormone. Here, SA treatment (200 μM) induced fungal resistance to HS of , with decreased malondialdehyde (MDA) content and expression. Further analysis showed that SA treatment in increased the cytosolic trehalose content and reduced the intracellular reactive oxygen species (ROS) level. Moreover, H O could restore the MDA content and expression of treated with SA under HS. In addition, trehalose (25 mM) or CaCl (5 mM) treatment induced fungal resistance to HS, and CaCl treatment increased the cytosolic trehalose content of under HS. However, inhibiting Ca levels using Ca inhibitors or mutants reversed the trehalose content induced by SA in under HS. In addition, inhibiting trehalose biosynthesis using -silenced strains reversed the MDA content and expression of treated with SA under HS. Taken together, these results indicate that SA treatment alleviates the HS response of by reducing the intracellular ROS level and increasing the cytosolic trehalose content. Heat stress (HS) is a crucial environmental challenge for edible fungi. Salicylic acid (SA), a plant defense-related hormone, plays key roles in plant responses to biotic and abiotic stresses. In this study, we found that SA treatment increased the cytosolic trehalose content and induced fungal resistance to HS in . Further analysis showed that SA can alleviate the HS of by reducing the intracellular ROS level and increasing the cytosolic trehalose content. Our results help to understand the mechanism underlying the responses of to HS. In addition, this research provides new insights for the cultivation of .