Principle and experiment of protein detection based on optical fiber sensing

A method for detecting protein molecules based on the tilted fiber Bragg grating (TFBG) surface plasma resonance (SPR) is proposed to achieve the quick online real-time detection of trace amount of proteins. The detection principles of the TFBG-SPR protein molecular probe are analyzed, and its feasi...

Full description

Saved in:
Bibliographic Details
Published in:Photonic sensors (Berlin) 2017-12, Vol.7 (4), p.317-324
Main Authors: Jiang, Qi, Xue, Mengwei, Liang, Pei, Zhang, Chengjia, Lin, Jianqiang, Ouyang, Jun
Format: Article
Language:English
Subjects:
Antigens
Cysteamine
Feasibility studies
Gold
Gratings (spectra)
Lasers
Measurement Science and Instrumentation
Microwaves
molecular probe
Optical Devices
Optics
Photonics
Physics
Physics and Astronomy
Plasma resonance
protein
Proteins
Regular
RF and Optical Engineering
Surface plasma resonance
TFBG
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for detecting protein molecules based on the tilted fiber Bragg grating (TFBG) surface plasma resonance (SPR) is proposed to achieve the quick online real-time detection of trace amount of proteins. The detection principles of the TFBG-SPR protein molecular probe are analyzed, and its feasibility is demonstrated. The intermediary material between the protein molecules and the golden layer outside of the fiber gratings is cysteamine hydrochloride. When the concentration of the cysteamine hydrochloride solution is 2 M, the shift of the TFBG resonance peak is 2.23 nm, illustrating that the cysteamine hydrochloride modifies the gold film successfully. IgG antigen solution is poured on the surface of the cysteamine hydrochloride modifying the gold-deposited TFBG. Finally, antigen-antibody hybridization experiment is carried out with a 10 mg/mL antibody solution, and after two hours of hybridization the resonance peak of the TFBG shifts 5.1 nm, which validates the feasibility and effectiveness of the TFBG-SPR protein molecular probe.
ISSN:1674-9251
2190-7439
DOI:10.1007/s13320-017-0383-7