Piper nigrum Leaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens

Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was charac...

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Published in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-9
Main Authors: Pandian, K., Malarkodi, Chelladurai, Rajeshkumar, Shanmugam, Vanaja, Mahendran, Gnanajobitha, Gnanadas, Paulkumar, Kanniah, Annadurai, Gurusamy
Format: Article
Language:English
Subjects:
Anti-Infective Agents - chemistry
Antibiotics
Antimicrobial agents
Aqueous solutions
Chemical properties
Chemical synthesis
Fourier transforms
Free radicals
Gold
Green chemistry
Herbal medicine
Host-Pathogen Interactions
Identification and classification
Infrared spectroscopy
Leaves
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Methods
Nanoparticles
Nanotechnology
Pathogens
Pepper
Permeability
Phenotype
Phytochemicals
Piper nigrum
Piper nigrum - chemistry
Piper nigrum - microbiology
Plant Diseases - microbiology
Plant Extracts - chemistry
Plant Leaves - chemistry
Plant Leaves - microbiology
Plant Stems - chemistry
Plant-pathogen relationships
Production processes
Silver
Silver - chemistry
Studies
Technology application
Ultraviolet spectroscopy
X-ray diffraction
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Annadurai, Gurusamy
description Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology.
doi_str_mv 10.1155/2014/829894
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In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. 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subjects Anti-Infective Agents - chemistry
Antibiotics
Antimicrobial agents
Aqueous solutions
Chemical properties
Chemical synthesis
Fourier transforms
Free radicals
Gold
Green chemistry
Herbal medicine
Host-Pathogen Interactions
Identification and classification
Infrared spectroscopy
Leaves
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Methods
Nanoparticles
Nanotechnology
Pathogens
Pepper
Permeability
Phenotype
Phytochemicals
Piper nigrum
Piper nigrum - chemistry
Piper nigrum - microbiology
Plant Diseases - microbiology
Plant Extracts - chemistry
Plant Leaves - chemistry
Plant Leaves - microbiology
Plant Stems - chemistry
Plant-pathogen relationships
Production processes
Silver
Silver - chemistry
Studies
Technology application
Ultraviolet spectroscopy
X-ray diffraction
title Piper nigrum Leaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens
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