Mesenchymal Stem Cell Conditioned Medium Modulates Inflammation in Tenocytes: Complete Conditioned Medium Has Superior Therapeutic Efficacy than Its Extracellular Vesicle Fraction

Tendinopathy, a prevalent overuse injury, lacks effective treatment options, leading to a significant impact on quality of life and socioeconomic burden. Mesenchymal stem/stromal cells (MSCs) and their secretome, including conditioned medium (CM) and extracellular vesicles (EVs), have shown promise...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (13), p.10857
Main Authors: Soukup, Robert, Gerner, Iris, Mohr, Thomas, Gueltekin, Sinan, Grillari, Johannes, Jenner, Florien
Format: Article
Language:English
Subjects:
Bone marrow
cell-free therapy
Chemokines
Culture Media, Conditioned - metabolism
Culture Media, Conditioned - pharmacology
Cytokines
equine mesenchymal stem cells (MSC)
Extracellular Vesicles - metabolism
Gene expression
Genes
Growth factors
Horses
Humans
Immunomodulation
Inflammation
Inflammation - metabolism
Ligands
Mesenchymal stem cells
Mesenchymal Stem Cells - metabolism
MSC extracellular vesicles (EVs)
MSC protein fraction
MSC secretome
Proteins
Proteins - metabolism
Quality of Life
Regeneration (physiology)
Secretome
Sports injuries
Stem cells
Stromal cells
Tendons
Tenocytes - metabolism
tenocytes regeneration
Tissue engineering
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Summary:Tendinopathy, a prevalent overuse injury, lacks effective treatment options, leading to a significant impact on quality of life and socioeconomic burden. Mesenchymal stem/stromal cells (MSCs) and their secretome, including conditioned medium (CM) and extracellular vesicles (EVs), have shown promise in tissue regeneration and immunomodulation. However, it remains unclear which components of the secretome contribute to their therapeutic effects. This study aimed to compare the efficacy of CM, EVs, and the soluble protein fraction (PF) in treating inflamed tenocytes. CM exhibited the highest protein and particle concentrations, followed by PF and EVs. Inflammation significantly altered gene expression in tenocytes, with CM showing the most distinct separation from the inflamed control group. Treatment with CM resulted in the most significant differential gene expression, with both upregulated and downregulated genes related to inflammation and tissue regeneration. EV treatment also demonstrated a therapeutic effect, albeit to a lesser extent. These findings suggest that CM holds superior therapeutic efficacy compared with its EV fraction alone, emphasizing the importance of the complete secretome in tendon injury treatment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241310857