In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigas

Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combination of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. The present article describes a competitive ELISA for the meas...

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Bibliographic Details
Published in:BMC immunology 2001-01, Vol.2 (1), p.1-1, Article 1
Main Authors: Torreilles, J, Romestand, B
Format: Article
Language:English
Subjects:
Amino compounds
Animals
Antibodies, Monoclonal - immunology
Bivalvia
Bivalvia - chemistry
Bivalvia - immunology
Bivalvia - metabolism
Blood proteins
Blood Proteins - chemistry
Crassostrea
Crassostrea gigas
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Hemocytes - metabolism
Measurement
Methods
Mytilus edulis
Mytilus galloprovincialis
Ostreidae - chemistry
Ostreidae - immunology
Ostreidae - metabolism
Oysters
Peroxynitrite
Peroxynitrous Acid - biosynthesis
Phagocytosis
Physiological aspects
Reference Standards
Tyrosine - analogs & derivatives
Tyrosine - analysis
Tyrosine - immunology
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Summary:Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combination of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG. This assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only. Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.
ISSN:1471-2172
1471-2172
DOI:10.1186/1471-2172-2-1