Isolation of Peptide Inhibiting SGC-7901 Cell Proliferation from Aspongopus chinensis Dallas

Aspongopus chinensis Dallas is used as a traditional Chinese medicine as well as an edible insect. Although its anti-tumor effects have been observed, the anti-tumor active component(s) in the hemolymph of A. chinensis remains unknown. In this study, a combination usage of ultrafiltration, gel filtr...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12535
Main Authors: Chen, Xu-Mei, Zhang, Shu-Qi, Cao, Mi-Lan, Guo, Jian-Jun, Luo, Rui
Format: Article
Language:English
Subjects:
Amino acids
anti-cancer peptide
Apoptosis
Aspongopus chinensis Dallas
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cell proliferation
Chemicals
Chinese medicine
Chromatography
Cloning
Drug dosages
Drug resistance
Esophagus
Gastric cancer
Gel filtration
Gene expression
Hemolymph
High performance liquid chromatography
IC50
inhibition
Leukemia
Liquid chromatography
Liver cancer
Lung cancer
Molecular weight
MTT
Peptides
Tumors
Ultrafiltration
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Summary:Aspongopus chinensis Dallas is used as a traditional Chinese medicine as well as an edible insect. Although its anti-tumor effects have been observed, the anti-tumor active component(s) in the hemolymph of A. chinensis remains unknown. In this study, a combination usage of ultrafiltration, gel filtration chromatography, FPLC and RP-HPLC to separate and purify active peptides was performed based on the proliferation of the human gastric cancer SGC-7901 cell line treated with candidates. One peptide (MW = 2853.3 Da) was isolated from the hemolymph of A. chinensis. A total of 24 amino acid residues were continuously determined for the active peptide: N′-ECGYCAEKGIRCDDIHCCTGLKKK-C′. In conclusion, a peptide that can inhibit the proliferation of gastric cancer SGC-7901 cells in the hemolymph of A. chinensis was purified in this study, which is homologous to members of the spider toxin protein family. These results should facilitate further works for this peptide, such as the cloning of genes, expression in vitro by prokaryotic or eukaryotic systems, more specific tests of anti-tumor activity, and so on.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232012535