Non-Affinity Purification of Antibodies

Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we p...

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Bibliographic Details
Published in:Antibodies (Basel) 2023-02, Vol.12 (1), p.15
Main Authors: Arakawa, Tsutomu, Tomioka, Yui, Nakagawa, Masataka, Sakuma, Chiaki, Kurosawa, Yasunori, Ejima, Daisuke, Tsumoto, Kouhei, Akuta, Teruo
Format: Article
Language:English
Subjects:
Acids
agarose
Antibodies
antibody
Ascites
Chemical properties
Chromatography
Column chromatography
Electrophoresis
Gel electrophoresis
Homogenization
Ligands
MEP
Methods
mixed-mode
Monoclonal antibodies
non-affinity
Protein A
Protein purification
Proteins
Purification
Resins
Review
Toxicity
Viral antibodies
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Summary:Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.
ISSN:2073-4468
2073-4468
DOI:10.3390/antib12010015