Direct Electron Transfer of Glucose Oxidase on Pre-Anodized Paper/Carbon Electrodes Modified through Zero-Length Cross-Linkers for Glucose Biosensors

A disposable paper-based glucose biosensor with direct electron transfer (DET) of glucose oxidase (GOX) was developed through simple covalent immobilization of GOX on a carbon electrode surface using zero-length cross-linkers. This glucose biosensor exhibited a high electron transfer rate (ks, 3.363...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-05, Vol.13 (5), p.566
Main Authors: Henao-Pabon, Gilberto, Gao, Ning, Prasad, K Sudhakara, Li, XiuJun
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Biosensors
Blood sugar
Carbon
Crosslinking
Dehydrogenases
Design and construction
Diabetes
Diabetes mellitus
direct electron transfer
electrochemical detection
Electrodes
Electron transfer
Electron transport
Electrons
Enzymatic activity
Enzymes, Immobilized
Glucose
glucose biosensors
Glucose Oxidase
glucose oxidase immobilization
Graphene
Immobilization
Measurement
Measuring instruments
Methods
microfluidic paper-based analytical device
Oxidation
Peptides
Potassium
Square waves
Testing
Voltammetry
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Summary:A disposable paper-based glucose biosensor with direct electron transfer (DET) of glucose oxidase (GOX) was developed through simple covalent immobilization of GOX on a carbon electrode surface using zero-length cross-linkers. This glucose biosensor exhibited a high electron transfer rate (ks, 3.363 s ) as well as good affinity (km, 0.03 mM) for GOX while keeping innate enzymatic activities. Furthermore, the DET-based glucose detection was accomplished by employing both square wave voltammetry and chronoamperometric techniques, and it achieved a glucose detection range from 5.4 mg/dL to 900 mg/dL, which is wider than most commercially available glucometers. This low-cost DET glucose biosensor showed remarkable selectivity, and the use of the negative operating potential avoided interference from other common electroactive compounds. It has great potential to monitor different stages of diabetes from hypoglycemic to hyperglycemic states, especially for self-monitoring of blood glucose.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13050566