Real‐time polymerase chain reaction quantification of the salivary levels of cariogenic bacteria in patients with orthodontic fixed appliances

Aim The aim was to investigate the salivary detection frequencies and quantities of caries‐associated bacteria from patients with orthodontic brackets. Methods Patients wearing orthodontic brackets (n = 40, mean age = 26 years) and healthy controls without brackets (n = 40, mean age = 17 years) were...

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Bibliographic Details
Published in:Clinical and experimental dental research 2020-06, Vol.6 (3), p.328-335
Main Authors: Al‐Melh, Manal A., Bhardwaj, Radhika G., Pauline, Eunice M., Karched, Maribasappa
Format: Article
Language:English
Subjects:
Bacteria
Binding sites
Dental plaque
Dentistry
Deoxyribonucleic acid
DNA
Enamel
Ethics
Laboratories
Oral hygiene
Original
orthodontic brackets
Orthodontics
Patients
plaque
Polymerase chain reaction
real‐time PCR
saliva
Sample size
Streptococcus infections
Streptococcus mutans
Streptococcus salivarius
Teeth
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Summary:Aim The aim was to investigate the salivary detection frequencies and quantities of caries‐associated bacteria from patients with orthodontic brackets. Methods Patients wearing orthodontic brackets (n = 40, mean age = 26 years) and healthy controls without brackets (n = 40, mean age = 17 years) were enrolled in the study. Saliva samples from each patient was collected. After DNA purification, target species comprising streptococci and a Lactobacillus species were detected and quantified from the samples using polymerase chain reaction (PCR) and real‐time quantitative PCR. Results Detection frequencies did not differ between the orthodontic patients and the control subjects for any target species except for Streptococcus sobrinus, which showed significantly lower detection rates in orthodontic patients (p 
ISSN:2057-4347
2057-4347
DOI:10.1002/cre2.285