Proteomics-based identification of haptoglobin as a favourable serum biomarker for predicting long-term response to splenectomy in patients with primary immune thrombocytopenia

Splenectomy is the most effective treatment for patients with primary immune thrombocytopenia (ITP) who fail to respond to steroid therapy. Thus far, there is no effective means to predict the long-term haematological response of the procedure. The purpose of this study was to identify serum biomark...

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Bibliographic Details
Published in:Journal of translational medicine 2012-10, Vol.10 (1), p.208-208, Article 208
Main Authors: Zheng, Chao-Xu, Ji, Zhuang-Qi, Zhang, Long-Juan, Wen, Qiong, Chen, Liu-Hua, Yu, Jun-Feng, Zheng, Dong
Format: Article
Language:English
Subjects:
Age
Analysis
Biomarkers
Biomarkers - blood
Blood platelets
Case-Control Studies
Drug therapy
Enzyme-Linked Immunosorbent Assay
Enzymes
Gel electrophoresis
Haptoglobin
Haptoglobins - metabolism
Humans
Immune response
Lasers
Mortality
Platelets
Primary immune thrombocytopenia
Proteins
Proteomics
Serum levels
Serum proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Splenectomy
Steroid hormones
Studies
Surgery
Thrombocytopenia
Thrombocytopenia - blood
Thrombocytopenia - immunology
Translation
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Summary:Splenectomy is the most effective treatment for patients with primary immune thrombocytopenia (ITP) who fail to respond to steroid therapy. Thus far, there is no effective means to predict the long-term haematological response of the procedure. The purpose of this study was to identify serum biomarkers as predictors of long-term response based on a proteomics approach. The serum samples of ITP patients were collected before splenectomy and seven days after surgery. After depletion of the abundant serum proteins, pooled preoperative serum samples from four responders to splenectomy, four nonresponders and four healthy controls were subjected to two-dimensional gel electrophoresis (2-DE). Nine protein spots with at least a five-fold alteration in expression between responders and nonresponders were all identified as haptoglobin (Hp) by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometer (MS) analysis. The validation of serum Hp expression was performed using enzyme-linked immunosorbent assays (ELISA) in thirty-seven responders, thirteen nonresponders and twenty-one healthy controls. The preoperative serum levels of Hp in the nonresponders (925.9 ± 293.5 μg/ml) were significantly lower than those in the responders (1417.4 ± 315.0 μg/ml, p
ISSN:1479-5876
1479-5876
DOI:10.1186/1479-5876-10-208