Class-Switching of B Lymphocytes by DNA and Cell Immunization for Stereospecific Monoclonal Antibodies against Native GPCR

To develop efficient applications of monoclonal antibodies for therapeutic purposes, stereospecific recognition of the target antigens is needed. DNA immunization is one of the best methods for sensitizing B lymphocytes that can produce conformation-specific antibodies. Here we verified the class-sw...

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Bibliographic Details
Published in:Immuno 2021-12, Vol.1 (4), p.432-441
Main Authors: Isozaki, Yushi, Tsumoto, Kanta, Tomita, Masahiro
Format: Article
Language:English
Subjects:
Antigens
B-cell receptor (BCR)
class-switching
DNA immunization
G protein-coupled receptor (GPCR)
Genes
Lymphocytes
Monoclonal antibodies
Multiple myeloma
native antigen
stereospecific monoclonal antibody
Vectors (Biology)
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Summary:To develop efficient applications of monoclonal antibodies for therapeutic purposes, stereospecific recognition of the target antigens is needed. DNA immunization is one of the best methods for sensitizing B lymphocytes that can produce conformation-specific antibodies. Here we verified the class-switching of monoclonal antibodies by DNA immunization followed by cell immunization for the generation of stereospecific monoclonal antibodies against native G protein-coupled receptor (GPCR) using the optimized stereospecific targeting (SST) technique. This technology facilitates the efficient selection of sensitized B lymphocytes through specific interaction with the intact antigen via B-cell receptors (BCRs). We demonstrate that multiple DNA immunizations followed by a single cell immunization in combination with a longer sensitization period (three to four months) are an appropriate sensitizing strategy for the generation of IgG-type stereospecific monoclonal antibodies by class-switching, and the characteristics of antibody production could be transferred to hybridoma cells provided by the optimized SST technique.
ISSN:2673-5601
2673-5601
DOI:10.3390/immuno1040031