Quantitative study of early-stage transient bacterial adhesion to bioactive glass and glass ceramics: atomic force microscopic observations

• Published in: Scientific reports 2024-09, Vol.14 (1), p.20336-13, Article 20336
• Main Authors: Gour, Shivani, Mukherjee, Abhijit, Balani, Kantesh, Dhami, Navdeep K.
• Format: Article
• Language: English
• Subjects: 631/326; 639/301; Adhesion; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Atomic force microscopy; Atomic force microscopy (AFM); Bacteria; Bacterial adhesion; Bacterial Adhesion - drug effects; Bio-mineral AFM probe; Bioactive glass; Biological activity; Ceramics; Ceramics - chemistry; E coli; Escherichia coli; Escherichia coli - drug effects; Force–distance measurement; Glass - chemistry; Gram-negative bacteria; Humanities and Social Sciences; Microscopy, Atomic Force - methods; multidisciplinary; Nanoparticles; Planktonic cells; Quantitative research; Science; Science (multidisciplinary); Staphylococcus aureus; Staphylococcus aureus - drug effects; Toxicity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-67716-0

Antimicrobial potential of bioactive glass (BAG) makes it promising for implant applications, specifically overcoming the toxicity concerns associated with traditional antibacterial nanoparticles. The 58S composition of BAG (with high Ca and absence of Na) has been known to exhibit excellent bioactivity and antibacterial behaviour, but the mechanisms behind have not been investigated in detail. In this pioneering study, we are using Atomic Force Microscopy (AFM) to gain insights into 58S BAG’s adhesive interactions with planktonic cells of both gram-positive ( Staphylococcus aureus ) and gram-negative ( Escherichia coli ) bacteria; along with the impact of crystallinity on antibacterial properties. We have recorded greater bacterial inhibition by amorphous BAG compared to semi-crystalline glass–ceramics and stronger effect against gram-negative bacteria via conventional long-term antibacterial tests. AFM force distance curves has illustrated substantial bonding between bacteria and BAG within the initial one second (observed at a gap of 250 ms) of contact, with multiple binding events. Further, stronger adhesion of BAG with E.coli (~ 6 nN) compared to S. aureus (~ 3 nN) has been found which can be attributed to more adhesive nano-domains (size effect) distributed uniformly on E.coli surface. This study has revealed direct evidence of impact of contact time and 58S BAG’s crystalline phase on bacterial adhesion and antimicrobial behaviour. Current study has successfully demonstrated the mode and mechanisms of initial bacterial adhesion with 58S BAG. The outcome can pave the way towards improving the designing of implant surfaces for a range of biomedical applications.