Humoral response in experimental autoimmune encephalomyelitis targets neural precursor cells in the central nervous system of naive rodents

• Published in: Journal of neuroinflammation 2017-11, Vol.14 (1), p.227-227, Article 227
• Main Authors: Kesidou, Evangelia, Touloumi, Olga, Lagoudaki, Roza, Nousiopoulou, Evangelia, Theotokis, Paschalis, Poulatsidou, Kyriaki-Nepheli, Boziki, Marina, Kofidou, Evangelia, Delivanoglou, Nickoleta, Minti, Fani, Hadjigeorgiou, Georgios, Grigoriadis, Nikolaos, Simeonidou, Constantina
• Format: Article
• Language: English
• Subjects: Affinity; Antigenic determinants; Antisera; Apoptosis; Autoantibodies; Autoantibody; Autoimmunity; Autophagy; B cells; Caspase-3; Central nervous system; Confocal microscopy; Cross-reactivity; Developmental stages; Disease; Doublecortin protein; Encephalomyelitis; Epitopes; Experimental allergic encephalomyelitis; Experimental autoimmune encephalomyelitis; Glial fibrillary acidic protein; Growth factors; Homeostasis; Immune response (humoral); Immunofluorescence; Immunoglobulins; Immunohistochemistry; Mediation; Mice; Multiple sclerosis; Musashi protein; Myelin; Myelin proteins; Myelination; Neonates; Nervous system; Neural precursor cells; Neurogenesis; Proteins; Remyelination; Rodents; Stem cells; Subventricular zone; Western blotting
• ISSN: 1742-2094; 1742-2094
• DOI: 10.1186/s12974-017-0995-2

Neural precursor cells (NPCs) located in the subventricular zone (SVZ), a well-defined NPC niche, play a crucial role in central nervous system (CNS) homeostasis. Moreover, NPCs are involved in the endogenous reparative process both in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). However, the possibility that NPCs may be vulnerable to immune-related components may not be ruled out. Therefore, we investigated the potential affinity of myelin oligodendrocyte glycoprotein (MOG)-induced humoral response(s) to NPCs. MOG -EAE was induced in C57BL/6 mice; blood-sampling was performed on days 17-21 (acute phase) along with a naive group and corresponding antisera (AS) were collected (EAE-AS, NAIVE-AS). The presence of anti-CNS autoantibodies was examined with western blotting. Furthermore, using the collected antisera and anti-MOG antibody (as positive control), immunohistochemistry and double immunofluorescence were implemented on normal neonatal, postnatal, and adult mouse brain sections. Targeted NPCs were identified with confocal microscopy. In vitro immunoreactivity assessment on NPCs challenged with autoantibodies was evaluated for apoptotic/autophagic activity. Western blotting verified the existence of autoantibodies in EAE mice and demonstrated bands corresponding to yet unidentified NPC surface epitopes. A dominant selective binding of EAE-AS in the subventricular zone in all age groups compared to NAIVE-AS (p