CRISPR-Cas and CRISPR-based screening system for precise gene editing and targeted cancer therapy

Target cancer therapy has been developed for clinical cancer treatment based on the discovery of CRISPR (clustered regularly interspaced short palindromic repeat) -Cas system. This forefront and cutting-edge scientific technique improves the cancer research into molecular level and is currently wide...

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Bibliographic Details
Published in:Journal of translational medicine 2024-05, Vol.22 (1), p.516-516, Article 516
Main Authors: Qin, Mingming, Deng, Chunhao, Wen, Liewei, Luo, Guoqun, Meng, Ya
Format: Article
Language:English
Subjects:
Animals
Cancer
Cancer therapy
CAR-T
Care and treatment
CRISPR screening
CRISPR-Cas system
CRISPR-Cas Systems - genetics
Gene Editing - methods
Genetic Therapy - methods
Humans
Molecular Targeted Therapy
Neoplasms - genetics
Neoplasms - therapy
Precision medicine
Review
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Summary:Target cancer therapy has been developed for clinical cancer treatment based on the discovery of CRISPR (clustered regularly interspaced short palindromic repeat) -Cas system. This forefront and cutting-edge scientific technique improves the cancer research into molecular level and is currently widely utilized in genetic investigation and clinical precision cancer therapy. In this review, we summarized the genetic modification by CRISPR/Cas and CRISPR screening system, discussed key components for successful CRISPR screening, including Cas enzymes, guide RNA (gRNA) libraries, target cells or organs. Furthermore, we focused on the application for CAR-T cell therapy, drug target, drug screening, or drug selection in both ex vivo and in vivo with CRISPR screening system. In addition, we elucidated the advantages and potential obstacles of CRISPR system in precision clinical medicine and described the prospects for future genetic therapy.In summary, we provide a comprehensive and practical perspective on the development of CRISPR/Cas and CRISPR screening system for the treatment of cancer defects, aiming to further improve the precision and accuracy for clinical treatment and individualized gene therapy.
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-024-05235-2