Transcriptome and genome-wide analysis of the mango glycosyltransferase family involved in mangiferin biosynthesis

• Published in: BMC genomics 2024-11, Vol.25 (1), p.1074-14, Article 1074
• Main Authors: Bai, Yibo, Huang, Xinran, Yao, Rundong, Zafar, Muhammad Mubashar, Chattha, Waqas Shafqat, Qiao, Fei, Cong, Hanqing
• Format: Article
• Language: English
• Subjects: Analysis; Anopheles; Antioxidants; Arabidopsis thaliana; Binding sites; Bioavailability; Biological activity; Biosynthesis; Chemical synthesis; Chromosomes; Developmental stages; Domestic relations; Evolutionary genetics; Flavonoids; Fruit - genetics; Fruit - metabolism; Fruits; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene-specific expression; Genes; Genome, Plant; Genomes; Genomic analysis; Genomics; Glycosides; Glycosylation; Glycosyltransferase; Glycosyltransferases - genetics; Glycosyltransferases - metabolism; Health aspects; Isoflavones; Localization; Mangifera - enzymology; Mangifera - genetics; Mangifera - metabolism; Mangoes; Metabolites; MiUGT; Molecular weight; Multigene Family; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; RNA; Tissues; Transcriptome; Transcriptomes; Xanthones - metabolism
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10998-5

Mangiferin, a C-glucosyl xanthone, is a biologically active glycoside naturally synthesized in mango. Glycosyltransferase can catalyze the biosynthesis of mangiferin. In this study, we identified 221 members of the UGT glycosyltransferase family in mango. The 221 MiUGT genes were grouped into 13 subfamilies through phylogenetic tree analysis with Arabidopsis, Chinese bayberry, and mango. All UGT family members in mango were unevenly distributed on 17 chromosomes and found that tandem duplication dominated the expansion of UGT family members in mango. Purification selection primarily influenced the evolution of the mango UGT family members. In addition, cis-element analysis of the mango UGT gene family revealed the presence of MYB binding sites, which are involved in flavonoid biosynthesis; which further supports the role of UGT family members in the synthesis of flavonoids. To verify these results, we analyzed the expression of UGT family members in mango leaves, stems, and different developmental stages of fruit peel. The RNA-seq and qRT-PCR results showed significant differences in the expression patterns of MiUGT genes in various tissues and developmental stages of mango. We identified MiUGT gene-specific expression at different stages of fruit development. These results lay a theoretical foundation for research on the relationship between members of the mango UGT family and the synthesis of flavonoids, mangiferin.