Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting

Thinopyrum intermedium possesses many desirable agronomic traits that make it a valuable genetic source for wheat improvement. The precise identification of individual chromosomes of allohexaploid Th. intermedium is a challenge due to its three sub-genomic constitutions with complex evolutionary anc...

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Bibliographic Details
Published in:Plants (Basel) 2022-08, Vol.11 (16), p.2109
Main Authors: Yu, Zhihui, Wang, Hongjin, Yang, Ennian, Li, Guangrong, Yang, Zujun
Format: Article
Language:English
Subjects:
Amphiploids
Chromatin
chromosome rearrangement
Chromosome rearrangements
Chromosomes
Fluorescence
Fluorescence in situ hybridization
Genetic aspects
Genomes
Hordeum vulgare
Hybridization
Identification
Karyotypes
ND-FISH
Oligo-FISH painting
Oligonucleotides
Physiological aspects
Plant breeding
Thinopyrum intermedium
Triticum aestivum
Wheat
Wheatgrasses
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Summary:Thinopyrum intermedium possesses many desirable agronomic traits that make it a valuable genetic source for wheat improvement. The precise identification of individual chromosomes of allohexaploid Th. intermedium is a challenge due to its three sub-genomic constitutions with complex evolutionary ancestries. The non-denaturing fluorescent in situ hybridization (ND-FISH) using tandem-repeat oligos, including Oligo-B11 and Oligo-pDb12H, effectively distinguished the St, J and JS genomes, while Oligo-FISH painting, based on seven oligonucleotide pools derived from collinear regions between barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.), was able to identify each linkage group of the Th. intermedium chromosomes. We subsequently established the first karyotype of Th. intermedium with individual chromosome recognition using sequential ND-FISH and Oligo-FISH painting. The chromosome constitutions of 14 wheat–Th. intermedium partial amphiploids and addition lines were characterized. Distinct intergenomic chromosome rearrangements were revealed among Th. intermedium chromosomes in these amphiploids and addition lines. The precisely defined karyotypes of these wheat–Th. intermedium derived lines may be helpful for further study on chromosome evolution, chromatin introgression and wheat breeding programs.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11162109