Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2024-03, Vol.14 (3), p.366
• Main Authors: Toader, Georgian Alin, Grigorean, Valentin Titus, Ionita, Mariana
• Format: Article
• Language: English
• Subjects: Adsorption; aminated graphene; Amino compounds; Analysis; Biosensors; Collodion; Contact angle; Deoxyribonucleic acid; DNA; DNA extraction; DNA, Single-Stranded; Experiments; Extraction (Chemistry); fluorescence quenching; Fourier transform infrared spectroscopy; Fourier transforms; Genetic testing; Graphene; Graphite - chemistry; Hydrophobicity; Infrared spectroscopy; ions; Magnesium chloride; Mechanical properties; Membranes; Methods; Morphology; Nanoparticles; Nitrocellulose; Nucleotides; Oligonucleotides; Oxides; Oxides - chemistry; Polyethylene glycol; Scanning electron microscopy; Scanning microscopy; Solid Phase Extraction; Spectrum analysis; Structure; Water
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14030366

A hybrid material, consisting of commercially available nitrocellulose (NC) membrane non-covalently modified with amino-polyethylene glycol functionalized reduced graphene oxide (NH -PEG-rGO) nanoparticles, was successfully synthesized for oligonucleotide extraction. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the modification of the NC membrane, revealing characteristic peaks of both compounds, i.e., NC and NH -PEG-rGO. Scanning Electron Microscopy (SEM) exhibited morphological changes in the NC/NH -PEG-rGO hybrid membrane, marked by the introduction of NH -PEG-rGO particles, resulting in a distinctly smothered surface compared to the porous surface of the NC control membrane. Wettability assays revealed hydrophobic behavior for the NC/NH -PEG-rGO hybrid membrane, with a water contact angle exceeding 90°, contrasting with the hydrophilic behavior characterized by a 16.7° contact angle in the NC membrane. The performance of the NC/NH -PEG-rGO hybrid membrane was evaluated for the extraction of ssDNA with fewer than 50 nucleotides from solutions containing various ionic species (MnCl , MgCl , and MnCl /MgCl ). The NC/NH -PEG-rGO hybrid membrane exhibited optimal performance when incubated in MgCl , presenting the highest fluorescence emission at 525 relative fluorescence units (r.f.u.). This corresponds to the extraction of approximately 610 pg (≈13%) of the total oligo-DNA, underscoring the efficacy of the pristine material, which extracts 286 pg (≈6%) of oligo-DNA in complex solutions.