Flavopereirine-An Alkaloid Derived from Geissospermum vellosii -Presents Leishmanicidal Activity In Vitro

• Published in: Molecules (Basel, Switzerland) Switzerland), 2019-02, Vol.24 (4), p.785
• Main Authors: da Silva E Silva, João Victor, Cordovil Brigido, Heliton Patrick, Oliveira de Albuquerque, Kelly Cristina, Miranda Carvalho, Josiwander, Ferreira Reis, Jordano, Vinhal Faria, Lara, Coelho-Ferreira, Márlia, Silveira, Fernando Tobias, da Silva Carneiro, Agnaldo, Percário, Sandro, do Rosário Marinho, Andrey Moacir, Dolabela, Maria Fâni
• Format: Article
• Language: English
• Subjects: Amphotericin B; apocynaceae; Binding sites; Bioavailability; Cell surface; Cytotoxicity; flavopereirine; Fractionation; Geissospermum vellosii; in silico modeling; Leishmania; medicinal chemistry; Molecular dynamics; Nifurtimox; Oligopeptidase; Oligopeptidase B; Ovaries; Parasites; Pharmacokinetics; Phosphopyruvate hydratase; Physicochemical properties; Phytochemicals; Prolyl oligopeptidase; Selectivity; Serine; Virulence
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24040785

Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of . In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC of 0.23 and 0.15 μg/mL, respectively). The extract, fractions, and flavopereirine presented low toxicity, with high selectivity for the alkaloid. Furthermore, flavopereirine showed no violation of Lipinski's rule of five, showing even better results than the known inhibitor of oligopeptidase B, antipain, with three violations. Flavopereirine also interacted with residue Tyr-499 of oligopeptidase B during the molecular dynamics simulations, giving a few insights of a possible favorable mechanism of interaction and a possible inhibitory pathway. Flavopereirine proved to be a promising molecule for its antileishmanial activity.