Diagnostic value of quantification of cell‐free DNA for suspected gallbladder cancer

• Published in: JGH open 2023-11, Vol.7 (11), p.748-754
• Main Authors: Sakamoto, Katsunori, Ogawa, Kohei, Tamura, Kei, Honjo, Masahiko, Sogabe, Kyosei, Ito, Chihiro, Iwata, Miku, Sakamoto, Akimasa, Shine, Mikiya, Nishi, Yusuke, Uraoka, Mio, Nagaoka, Tomoyuki, Funamizu, Naotake, Takada, Yasutsugu
• Format: Article
• Language: English
• Subjects: Antigens; Apoptosis; carcinoembryonic antigen; cell‐free DNA; Disease; Gallbladder cancer; Gallbladder diseases; Original; Patients; Software; tumor depth
• ISSN: 2397-9070; 2397-9070
• DOI: 10.1002/jgh3.12977

Background and Aim An accurate preoperative diagnosis as the basis for deciding the most appropriate surgical procedure is essential for patients with suspected gallbladder cancer (GBC). The aim of this study was to investigate the usefulness of cell‐free DNA (cfDNA) for the preoperative detection of ≥T2 invasion in patients with suspected GBC. Methods Twenty‐four patients who underwent resection for suspected GBC were enrolled. The concentration of cfDNA obtained from blood samples preoperatively was measured and evaluated in two distributions. The first peak (less than 200 base pairs) of cfDNA distribution was defined as the shorter fragment cfDNA, considered to originate mainly from apoptosis; and the second peak (200 base pairs or more) was defined as the longer fragment cfDNA, originating mainly from necrosis. Results Pathological analysis identified benign disease in 12 patients and GBC in 12 patients, of whom 6 patients had ≥pT2 GBC. Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19‐9 were significantly higher in the ≥pT2 GBC group than in the benign/