Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure

• Published in: Microorganisms (Basel) 2023-05, Vol.11 (6), p.1362
• Main Authors: Bao, Xu-Chong, Tang, Hong-Zhi, Li, Xue-Gong, Li, An-Qi, Qi, Xiao-Qing, Li, Deng-Hui, Liu, Shan-Shan, Wu, Long-Fei, Zhang, Wei-Jia
• Format: Article
• Language: English
• Subjects: Adaptation; Bacteria; Biodiversity and Ecology; Bioluminescence; Deep sea; Deep sea environments; deep-sea bacterium; DNA repair; E coli; Environmental Sciences; Enzymes; high hydrostatic pressure; Hydrostatic pressure; Intracellular; Metabolism; Microorganisms; Mutants; Oxidation; Oxidative stress; Photobacterium phosphoreum; Physiology; Pressure tolerance; Reactive oxygen species; Scavenging; Strain
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11061362

Bioluminescence is a common phenomenon in nature, especially in the deep ocean. The physiological role of bacterial bioluminescence involves protection against oxidative and UV stresses. Yet, it remains unclear if bioluminescence contributes to deep-sea bacterial adaptation to high hydrostatic pressure (HHP). In this study, we constructed a non-luminescent mutant of Δ and its complementary strain c-Δ of ANT-2200, a deep-sea piezophilic bioluminescent bacterium. The wild-type strain, mutant and complementary strain were compared from aspects of pressure tolerance, intracellular reactive oxygen species (ROS) level and expression of ROS-scavenging enzymes. The results showed that, despite similar growth profiles, HHP induced the accumulation of intracellular ROS and up-regulated the expression of ROS-scavenging enzymes such as , and , specifically in the non-luminescent mutant. Collectively, our results suggested that bioluminescence functions as the primary antioxidant system in strain ANT-2200, in addition to the well-known ROS-scavenging enzymes. Bioluminescence contributes to bacterial adaptation to the deep-sea environment by coping with oxidative stress generated from HHP. These results further expanded our understanding of the physiological significance of bioluminescence as well as a novel strategy for microbial adaptation to a deep-sea environment.