Development of an RNA Nanostructure for Effective Botrytis cinerea Control through Spray-Induced Gene Silencing without an Extra Nanocarrier

Spray-induced gene silencing represents an eco-friendly approach for crop protection through the use of double-stranded RNA (dsRNA) to activate the RNA interference (RNAi) pathway, thereby silencing crucial genes in pathogens. The major challenges associated with dsRNA are its limited stability and...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2024-07, Vol.10 (7), p.483
Main Authors: Wu, Fangli, Yan, Ling, Zhao, Xiayang, Lv, Chongrun, Jin, Weibo
Format: Article
Language:English
Subjects:
Aqueous solutions
Bioassays
Botrytis cinerea
Comparative analysis
Design
Disease control
Double-stranded RNA
E coli
Enzymes
Fungi
fungicide
Gene expression
Gene silencing
Genes
Genetic engineering
Microorganisms
nanocarrier free
Nanoparticles
Pathogens
Phenols
Plant diseases
Plant protection
RNA
RNA nanoparticle
RNA-mediated interference
SIGS
siRNA
Spore germination
Virulence
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Summary:Spray-induced gene silencing represents an eco-friendly approach for crop protection through the use of double-stranded RNA (dsRNA) to activate the RNA interference (RNAi) pathway, thereby silencing crucial genes in pathogens. The major challenges associated with dsRNA are its limited stability and poor cellular uptake, necessitating repeated applications for effective crop protection. In this study, RNA nanoparticles (NPs) were proposed as effectors in plants and pathogens by inducing the RNAi pathway and silencing gene expression. RNA structural motifs, such as hairpin-loop, kissing-loop, and tetra-U motifs, were used to link multiple siRNAs into a long, single-stranded RNA (lssRNA). The lssRNA, synthesized in , self-assembled into stable RNA nanostructures via local base pairing. Comparative analyses between dsRNA and RNA NPs revealed that the latter displayed superior efficacy in inhibiting spore germination and mycelial growth of . Moreover, RNA NPs had a more robust protective effect on plants against than did dsRNA. In addition, RNA squares are processed into expected siRNA in plants, thereby inhibiting the expression of the target gene. These findings suggest the potential of RNA NPs for use in plant disease control by providing a more efficient and specific alternative to dsRNA without requiring nanocarriers.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof10070483