Inhibition of miR-199b-5p reduces pathological alterations in osteoarthritis by potentially targeting Fzd6 and Gcnt2

Osteoarthritis (OA) is a degenerative disease with a high prevalence in the elderly population, but our understanding of its mechanisms remains incomplete. Analysis of serum exosomal small RNA sequencing data from clinical patients and gene expression data from OA patient serum and cartilage obtaine...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2024-05, Vol.12
Main Authors: Feng, Tong, Zhang, Qi, Li, Si-Hui, Ping, Yan-Ling, Tian, Mu-Qiu, Zhou, Shuan-Hu, Wang, Xin, Wang, Jun-Meng, Liang, Fan-Rang, Yu, Shu-Guang, Wu, Qiao-Feng
Format: Article
Language:English
Subjects:
animal
Animals
Arthritis
Bioinformatics
Biosynthesis
Cartilage
Cartilage diseases
Chondrocytes
Chondrocytes - metabolism
Chromosome 5
Collagen
Disease Models, Animal
Enzymes
Extracellular matrix
Frizzled Receptors - genetics
Frizzled Receptors - metabolism
Gene expression
Gene Expression Regulation
Genes
Genomes
Humans
Immunology and Inflammation
Inflammation
Joint replacement surgery
Kinases
Male
Metabolism
Mice
Mice, Inbred C57BL
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
miRNA-199b-5p
Ontology
Osteoarthritis
Osteoarthritis - genetics
Osteoarthritis - metabolism
Osteoarthritis - pathology
Patients
RNA polymerase
Therapeutic targets
Transcription factors
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Osteoarthritis (OA) is a degenerative disease with a high prevalence in the elderly population, but our understanding of its mechanisms remains incomplete. Analysis of serum exosomal small RNA sequencing data from clinical patients and gene expression data from OA patient serum and cartilage obtained from the GEO database revealed a common dysregulated miRNA, miR-199b-5p. In vitro cell experiments demonstrated that miR-199b-5p inhibits chondrocyte vitality and promotes extracellular matrix degradation. Conversely, inhibition of miR-199b-5p under inflammatory conditions exhibited protective effects against damage. Local viral injection of miR-199b-5p into mice induced a decrease in pain threshold and OA-like changes. In an OA model, inhibition of miR-199b-5p alleviated the pathological progression of OA. Furthermore, bioinformatics analysis and experimental validation identified and as potential target genes of . Thus, these results indicated that / and axis might be a novel therapeutic target for the treatment of OA.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.92645