Image analysis of Moroccan carob seeds (Ceratonia siliqua L.) revealed substantial intraspecific variations depending on climate and geographic origin

• Published in: Ecological processes 2022-04, Vol.11 (1), p.1-14, Article 34
• Main Authors: Kassout, Jalal, Hmimsa, Younes, El Fatehi, Salama, El Ouahrani, Abdeltif, Kadaoui, Khalil, Chakkour, Soufian, Ariza-Mateos, David, Palacios-Rodríguez, Guillermo, Navarro-Cerrillo, Rafael, Ater, Mohammed
• Format: Article
• Language: English
• Subjects: Agricultural ecosystems; Amplitude; Amplitudes; Analysis; Area; Aspect ratio; Bioclimatology; Carob; Carob tree; Ceratonia siliqua; Climate; Climatic conditions; Earth and Environmental Science; Ecological conditions; Economics; Environment; Environmental changes; Genetic diversity; Genetic variation; Image analysis; Image processing; Intraspecific variation; Morocco; Morphometry; Plant species; Populations; Roundness; Seed traits; Seeds; Shape; Trees; Variability
• ISSN: 2192-1709; 2192-1709
• DOI: 10.1186/s13717-022-00378-w

Background The carob tree ( Ceratonia siliqua L.) is one of the most iconic tree species of the Mediterranean region, with valuable economic, ecological and cultural value. Carob has been exploited around the Mediterranean region since antiquity and has been regarded as an important component of natural habitats and traditional agroecosystems. Several studies have focused on its morphological, biochemical, and genetic diversity. However, less is known about the intraspecific variation of seed traits. In this regard, and as an overall objective, we intend to evaluate the amplitude and the expression of intraspecific variations of carob seed traits at different ecological scales ranging from individual trees to different geographical landscapes. In addition, we investigated how the climate along the study area affects the extent of carob seed variability. Using image analysis techniques, we measured seven traits related to the size and the shape of 1740 seeds collected from 18 populations of spontaneous C. siliqua distributed along a latitudinal transect in Morocco under different bioclimatic conditions. Results The morphometric analysis of carob seed showed the effectiveness of adopted approach to highlight the amount and the amplitude of intraspecific variation according to geographic and climatic factors. Seed trait analysis revealed high intraspecific variability, explained by differences between and among carob populations and geographic zones. Seed area, perimeter, length, and width showed the largest variability between geographic zones. However, circularity, aspect ratio, and seed roundness showed higher variability at the tree level. Finally, our results show that seed traits vary depending on altitude and climate condition. Conclusions Revealing the amount and the structure of intraspecific traits variability of carob seed provides interesting insights to understand the mechanisms underlying trees adaptation to various environmental and ecological conditions. Therefore, intraspecific variation of seed traits should be integrated into trait-based functional ecology to assess plant species responses to environmental changes.