Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age

Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanc...

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Bibliographic Details
Published in:International journal of genomics 2019-01, Vol.2019 (2019), p.1-9
Main Authors: Fornal, A., Gurgul, A., Szmatoła, T., Semik-Gurgul, E., Ząbek, T., Bugno-Poniewierska, M.
Format: Article
Language:English
Subjects:
Aging
Bisulfite
Cancer
Deoxyribonucleic acid
DNA
DNA methylation
DNA sequencing
Epigenetics
Gene expression
Genomes
Genomics
Horses
Immune response
Immune system
Leukocytes
Middle age
Neurogenesis
Transduction
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Summary:Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.
ISSN:2314-436X
2314-4378
DOI:10.1155/2019/2839614