Oxidative stress‐induced changes in the transcriptomic profile of extracellular vesicles

Extracellular vesicles (EVs) have been proposed to play dual roles in cellular homeostasis, functioning both to remove unwanted intracellular molecules, and to enable communication between cells as a means of modulating cellular responses in different physiological and pathological scenarios. EVs co...

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Bibliographic Details
Published in:Journal of extracellular biology 2024-04, Vol.3 (4), p.e150-n/a
Main Authors: Dellar, Elizabeth R., Hill, Claire, Carter, David R. F., Baena‐Lopez, Luis Alberto
Format: Article
Language:English
Subjects:
Drosophila
ExRNA
extracellular vesicles
oxidative stress
RNA
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Summary:Extracellular vesicles (EVs) have been proposed to play dual roles in cellular homeostasis, functioning both to remove unwanted intracellular molecules, and to enable communication between cells as a means of modulating cellular responses in different physiological and pathological scenarios. EVs contain a broad range of cargoes, including multiple biotypes of RNA, which can vary depending on the cell status, and may function as signalling molecules. In this study, we carried out comparative transcriptomic analysis of Drosophila EVs and cells, demonstrating that the RNA profile of EVs is distinct from cells and shows dose‐dependent changes in response to oxidative stress. We identified a high abundance of snoRNAs in EVs, alongside an enrichment of intronic and untranslated regions (UTRs) of mRNAs under stress. We also observed an increase in the relative abundance of either aberrant or modified mRNAs under stress. These findings suggest that EVs may function both for the elimination of specific cellular RNAs, and for the incorporation of RNAs that may hold signalling potential.
ISSN:2768-2811
2768-2811
DOI:10.1002/jex2.150