Ceftazidime/Tobramycin Co-Loaded Chitosan-Coated Zein Nanoparticles against Antibiotic-Resistant and Biofilm-Producing Pseudomonas aeruginosa and Klebsiella pneumoniae

This study aimed to co-encapsulate ceftazidime and tobramycin in zein nanoparticles coated with chitosan and to characterize and evaluate the antibacterial and antibiofilm activity against antibiotic-resistant and . Zein nanoparticles, synthesized using the nanoprecipitation method, were characteriz...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-02, Vol.17 (3), p.320
Main Authors: Campos, Luís André de Almeida, Neto, Azael Francisco Silva, Scavuzzi, Alexsandra Maria Lima, Lopes, Ana Catarina De Souza, Santos-Magalhães, Nereide Stela, Cavalcanti, Isabella Macário Ferro
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
Bacterial infections
bacterial resistance
Bioavailability
Biofilms
combination therapy
COVID-19
Cystic fibrosis
Drug resistance
infections
Mortality
nanocarriers
Nanoparticles
Nosocomial infections
Pneumonia
Polymers
Scanning electron microscopy
Spectrum analysis
Vibration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to co-encapsulate ceftazidime and tobramycin in zein nanoparticles coated with chitosan and to characterize and evaluate the antibacterial and antibiofilm activity against antibiotic-resistant and . Zein nanoparticles, synthesized using the nanoprecipitation method, were characterized by their particle size (Ø), polydispersity index (PDI), zeta potential (ζ), pH, and encapsulation efficiency (%EE). The chitosan coating provided stability, and physicochemical analyses revealed chemical interactions, efficient drug encapsulation, and thermal stability. The release kinetics demonstrated controlled release in simulated gastric and intestinal pH. The antibacterial activity, assessed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), indicated effectiveness against both pathogens. Antibiofilm assays, conducted using the crystal violet method, demonstrated the inhibition and eradication of biofilms. The chitosan-coated zein nanoparticles with CAZ and/or TOB exhibited Ø (315-335 nm), PDI (55%). Notably, the co-encapsulation formulation (CAZ-TOB-ZNP-CH) showed enhanced antibacterial and antibiofilm activities compared to the individual formulations. These findings suggest that the developed nanoparticles present a promising alternative for treating respiratory and intestinal infections caused by antibiotic-resistant and biofilm-producing and .
ISSN:1424-8247
1424-8247
DOI:10.3390/ph17030320