Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model

PURPOSE:To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model.METHODS: Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wo...

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Published in:Acta cirúrgica brasileira 2014-09, Vol.29 (9), p.596-602
Main Authors: Almeida, Bernadeth Moda de, Nascimento, Marismar Fernandes do, Pereira-Filho, Rose Nely, Melo, Genecy Calado de, Santos, José Cleveilton dos, Oliveira, Clauberto Rodrigues de, Gomes, Margarete Zanardo, Lima, Sônia Oliveira, Albuquerque-Júnior, Ricardo Luiz Cavalcanti de
Format: Article
Language:English
Subjects:
Histological Techniques
Immunohistochemistry
Rats, Wistar
Wound Healing
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Summary:PURPOSE:To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model.METHODS: Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wounded histologically processed to assess the immunohistochemical expression of CD3, CD20, CD31, α-SMA and type-I collagen. Non-injured skin samples (NSS) were used as control. Data were subjected to statistical analysis using ANOVA and Tukey test.RESULTS: The mean of CD3 and CD20 positive cells in the wounds was significantly higher than in NSS at seven and 14 days (p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-8650201400150007