Astigmatic traction force microscopy (aTFM)

Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information...

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Bibliographic Details
Published in:Nature communications 2021-04, Vol.12 (1), p.2168-2168, Article 2168
Main Authors: Li, Di, Colin-York, Huw, Barbieri, Liliana, Javanmardi, Yousef, Guo, Yuting, Korobchevskaya, Kseniya, Moeendarbary, Emad, Li, Dong, Fritzsche, Marco
Format: Article
Language:English
Subjects:
14/63
631/1647/2204
631/1647/245
631/1647/245/2225
631/57
Animals
Biomechanical Phenomena
Calibration
Cell Adhesion
Fluorescence
Fluorescence microscopy
Force measurement
HeLa Cells
Humanities and Social Sciences
Humans
Microscopy
Microscopy, Atomic Force
multidisciplinary
Rats
Science
Science (multidisciplinary)
Sensitivity
Temporal resolution
Traction
Traction force
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Summary:Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity. Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-22376-w