A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light. Although there are many bioluminescent systems, few...

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Bibliographic Details
Published in:Biochemistry research international 2024-02, Vol.2024, p.8273237-22
Main Authors: Dunuweera, Asiri N., Dunuweera, Shashiprabha P., Ranganathan, K.
Format: Article
Language:English
Subjects:
Bacteria
Bioluminescence
Biomedical materials
Biosynthesis
Chemical bonds
Drug discovery
Enzymes
Immunoassay
Industrial applications
Light
Light emission
Luciferase
Luciferin
Medical research
Medicine, Experimental
Microorganisms
Organisms
Oxidation
Protein interaction
Protein-protein interactions
Proteins
Research projects
Review
Technology application
Wavelengths
Citations: Items that this one cites
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Summary:Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light. Although there are many bioluminescent systems, few have been identified. D-luciferin-dependent systems, coelenterazine-dependent systems, Cypridina luciferin-based systems, tetrapyrrole-based luciferins, bacterial bioluminescent systems, and fungal bioluminescent systems are natural bioluminescent systems. Since different bioluminescence systems, such as various combinations of luciferin-luciferase pair reactions, have different light emission wavelengths, they benefit industrial applications such as drug discovery, protein-protein interactions, in vivo imaging in small animals, and controlling neurons. Due to the expression of luciferase and easy permeation of luciferin into most cells and tissues, bioluminescence assays are applied nowadays with modern technologies in most cell and tissue types. It is a versatile technique in a variety of biomedical research. Furthermore, there are some investigated blue-sky research projects, such as bioluminescent plants and lamps. This review article is mainly based on the theory of diverse bioluminescence systems and their past, present, and future applications.
ISSN:2090-2247
2090-2255
DOI:10.1155/2024/8273237